Molecular evidence that the proliferative kidney disease organism unknown (PKX) is a myxosporean
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Molecular evidence that the proliferative kidney disease organism unknown (PKX) is a myxosporean.
The proliferative kidney organism unknown (PKX), a serious salmonid fish pathogen, is considered to be a myxosporean on the basis of ultrastructural studies, but its real taxonomic position has never been confirmed. In order to ascertain its position, genomic DNA was extracted from PKX and small subunit (SSU) ribosomal DNA was amplified by PCR, cloned and sequenced. A phylogenetical analysis on...
متن کاملProliferative kidney disease in brown trout Salmo trutta: further evidence of a myxosporean aetiology
Two groups of brown trout Salmo trutta L. were studied, one after natural exposure to PKD-infected waters, the other after laboratory challenge by intraperitoneal inoculation with PKDinfected kidney tissue from rainbow trout Salmo gairdneri Richardson. Subclinical PKD developed in both groups; renal intraluminal myxosporean organisms were observed in both groups. The implications of these findi...
متن کاملObservations on the electron-dense bodies of the PKX parasite, agent of proliferative kidney disease in salmonids.
Ultrastructural observations on structures associated with the 'haplosporosome'-like electron-dense bodies (EDBs) of the primary cell of the extrasporogonic stage of PKX are described. Observations made include formation of EDBs by the trans-Golgi network, an additional membrane associated with EDB structure, confronting cisternae, engulfment and presence of EDBs in multivesicular bodies, fusio...
متن کاملNON-SALMONID FISH INHABITING A HATCHERY WATER SUP- PLY ENZOOTIC FOR PROLIFERATIVE KIDNEY DISEASE IN- FECTED WITH ONE MYXOSPOREAN, Myxobilatus SP'P
Proliferative kidney disease (PKD), caused by an unclassified myxosporean (PKX), is a serious disease of salmonids. Although partial spore development occurs to a limited extent in fish infected with PKX, complete sporalation has not been observed and precise taxonomic status of PKX remains uncertain. The severe inflammation seen in infected fish, unusual for most myxosporean infections, sugges...
متن کاملRapid lectin-based staining procedure for the detection of the myxosporean causing proliferative kidney disease in salmonid fish
Lectins are proteins found initially in plants but now known from viruses, protozoans and h ~ g h e r animals which recognize specific glycoconjugates on cell surfaces. A rapid staining procedure using the lectin GS-1 from Griffonia simplicifolia was developed for the detect~on of PKX, the myxosporean causing prol~ferative kidney disease (PKD) in salmonid fish. A single developmental (extraspor...
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ژورنال
عنوان ژورنال: Diseases of Aquatic Organisms
سال: 1999
ISSN: 0177-5103,1616-1580
DOI: 10.3354/dao036209